Plant Breeding, Genetic Modification, Genome Editing (GATE XL) — Stud…
Principles, methods – selection, hybridization, heterosis; Male sterility; Genetic maps and molecular markers; Embryo rescue; haploid and doubled haploids; Plant tissue culture: micropropagation, embryo culture and in vitro regeneration, somatic embryogenesis, artificial seed, cryopreservation, somaclonal variation, somatic cell hybridization, marker-assisted selection, gene transfer methods viz. direct and vector-mediated, generation of transgenic plants; Introduction to genome editing: CRISPR/Cas9, Cre-Lox system to generate chimeras; Plastid transformation; chemical mutagenesis.