Analytical Techniques (GATE XL) — Study Resources & PYQs
Buffering against pH changes in biological systems, the Henderson-Hasselbalch equation; Ion exchange, size exclusion and affinity chromatography, Thin layer and paper chromatography, centrifugation; Characterization of biomolecules by electrophoresis; Analytical methods to study DNA-protein and protein–protein interactions; Lambert-Beer law, UV-visible, and fluorescence spectroscopy; next-generation DNA sequencing, PCR, and site-directed mutagenesis. Antibody-antigen interaction; Immunological techniques: immunodiffusion, immune-electrophoresis, RIA and ELISA, flow cytometry; monoclonal antibodies and their applications.